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denaturing gradient gel electrophoresis (DGGE) 3

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N-glycans 2

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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Engineering doi: 10.1016/j.eng.2023.02.016

Abstract: chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillarygel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gelelectrophoresis     Mass spectrometry    

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Relative writing age analysis of the black roller pen inksby capillary zone electrophoresis

Wang Yanji,Zhao Pengcheng,Xu Yuanyuan, Wan Jinghan

Strategic Study of CAE 2007, Volume 9, Issue 2,   Pages 25-29

Abstract:

 The analytical method for roller pen inks was established by capillaryzone electrophoresis.After optimizing such conditions as20mmol桙L borate buffer at pH 8.5, 100μm×40cm fused silica capillary column,separation voltage of15kV,photo diode array (PDA) detector and scanning

Keywords： black roller pen inks     zone capillary electrophoresis     writin    

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Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Engineering 2023, Volume 26, Issue 7,   Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

Abstract: chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillarygel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillarygel electrophoresis     Mass spectrometry    

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Application of proteomics in environmental science

Xiaona CHU, Jiangyong HU, Say Leong ONG,

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 4,   Pages 393-403 doi: 10.1007/s11783-009-0145-x

Abstract: article provides a review on the development of the main proteomic technologies, including both the gelbased and non-gel based technologies, and their applications in environmental science.

Keywords： proteomics     environmental stress     two dimensional (2D) gel electrophoresis     mass spectrometry    

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Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 82-97 doi: 10.1007/s11783-011-0306-6

Abstract: Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

Keywords： molecular biological technique     microbial community     denaturing gradient gel electrophoresis (DGGE)     terminal    

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Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 196-205 doi: 10.1007/s11684-017-0560-5

Abstract:

We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE

Keywords： Clostridium difficile     multiplex PCR     capillary electrophoresis     detection     characterization    

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Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 40-47 doi: 10.1007/s11783-011-0304-8

Abstract: analyze the microbial community shift along with environmental variations using denaturing gradient gelelectrophoresis of polymerase chain reaction amplified 16S rDNA fragments.

Keywords： sewage treatment plants     polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE    

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Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords： quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

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PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China

Wei Li, Shan Lu, Zhigang Cui, Jinghua Cui, Haijian Zhou, Yiqing Wang, Zhujun Shao, Changyun Ye, Biao Kan, Jianguo Xu

Frontiers of Medicine 2012, Volume 6, Issue 4,   Pages 366-375 doi: 10.1007/s11684-012-0214-6

Abstract:

Surveillance is critical for the prevention and control of infectious disease. China’s real-time web-based infectious disease reporting system is a distinguished achievement. However, many aspects of the current China Infectious Disease Surveillance System do not yet meet the demand for timely outbreak detection and identification of emerging infectious disease. PulseNet, the national molecular typing network for foodborne disease surveillance was first established by the Centers for Disease Control and Prevention of the United States in 1995 and has proven valuable in the early detection of outbreaks and tracing the pathogen source. Since 2001, the China CDC laboratory for bacterial pathogen analysis has been a member of the PulseNet International family; and has been adapting the idea and methodology of PulseNet to develop a model for a future national laboratory-based surveillance system for all bacterial infectious disease. We summarized the development progress for the PulseNet China system and discussed it as a model for the future of China’s national laboratory-based surveillance system.

Keywords： infectious disease     laboratory-based infectious disease surveillance     pulse field gel electrophoresis     multilocus    

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Simulation on refrigerant flow in adiabatic capillary tube

WANG Meixia, LIU Cunfang, ZHOU Qiangtai

Frontiers of Mechanical Engineering 2008, Volume 3, Issue 3,   Pages 332-336 doi: 10.1007/s11465-008-0054-x

Abstract: This paper proposes a new mathematical model to calculate flow characteristics of the adiabatic capillary

Keywords： mathematical     diameter     literature     sub-cooling     adiabatic capillary    

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Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 2,   Pages 324-333 doi: 10.1007/s11783-014-0627-3

Abstract: community structure of the granules was also analyzed by polymerase chain reaction-denaturing gradient gelelectrophoresis (PCR-DGGE).

Keywords： CMAS)     continuous flow     shear force     filamentous bacteria     polymerase chain reaction-denaturing gradient gelelectrophoresis (PCR-DGGE)    

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Visual chiral recognition of 1,1′-binaphthol through enantioselective collapse of gel based on an amphiphilic

Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 2,   Pages 231-237 doi: 10.1007/s11705-017-1633-3

Abstract: The resulting gel can be applied as a fascinating platform for visual recognition of enantiomeric 1-(2-hydroxynaphthalen-1-yl)naphthalen-2-ol (BINOL) through selective gel collapse.In addition, the mechanism for the reaction of the gel with chiral BINOL was investigated by scanning

Keywords： gelator     Schiff base     chiral recognition     gel formation     gel collapse    

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Microstructural and photocatalytic characterization of cement-paste sol-gel synthesized titanium dioxide

Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO

Frontiers of Structural and Civil Engineering 2016, Volume 10, Issue 2,   Pages 189-197 doi: 10.1007/s11709-015-0326-6

Abstract: containing calcium silicate hydrate (C-H-S) particles are identified as networking centers of a C-S-H gelnew cement matrix may be twofold advantageous since it additionally promotes the formation of C-S-H gel

Keywords： cement composites     photocatalytic TiO2     sol-gel     C-S-H gel     microstructure    

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A concept of capillary active, dynamic insulation integrated with heating, cooling and ventilation, air

Mark BOMBERG

Frontiers of Structural and Civil Engineering 2010, Volume 4, Issue 4,   Pages 431-437 doi: 10.1007/s11709-010-0071-9

Abstract: It is focused on the most successful applications of capillary active, dynamic interior thermal insulationStarting with a pioneering work of the Technical University in Dresden in development of capillary active

Keywords： capillary active insulation     integrated heating     cooling and ventilation     air conditioning (HVAC) and building    

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Available energy analysis of new tandem double-capillary tube refrigeration system for refrigerator-freezers

HE Maogang, ZHANG Ying, SONG Xinzhou, ZHANG Jiantao

Frontiers in Energy 2008, Volume 2, Issue 1,   Pages 36-42 doi: 10.1007/s11708-008-0013-6

Abstract: A new tandem double-capillary tube refrigeration system for refrigerator-freezers is proposed.A capillary tube was added between the two evaporators in the fresh and frozen food storage chests tois 21.20% and 20.57%, respectively when the refrigerant is R12 and R134a, while that of the double-capillary
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Title Author Date Type Operation

Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Journal Article

Relative writing age analysis of the black roller pen inksby capillary zone electrophoresis

Wang Yanji,Zhao Pengcheng,Xu Yuanyuan, Wan Jinghan

Journal Article

Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Journal Article

Application of proteomics in environmental science

Xiaona CHU, Jiangyong HU, Say Leong ONG,

Journal Article

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Journal Article

Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Journal Article

Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article

PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China

Wei Li, Shan Lu, Zhigang Cui, Jinghua Cui, Haijian Zhou, Yiqing Wang, Zhujun Shao, Changyun Ye, Biao Kan, Jianguo Xu

Journal Article

Simulation on refrigerant flow in adiabatic capillary tube

WANG Meixia, LIU Cunfang, ZHOU Qiangtai

Journal Article

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

Journal Article

Visual chiral recognition of 1,1′-binaphthol through enantioselective collapse of gel based on an amphiphilic

Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu

Journal Article

Microstructural and photocatalytic characterization of cement-paste sol-gel synthesized titanium dioxide

Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO

Journal Article

A concept of capillary active, dynamic insulation integrated with heating, cooling and ventilation, air

Mark BOMBERG

Journal Article

Available energy analysis of new tandem double-capillary tube refrigeration system for refrigerator-freezers

HE Maogang, ZHANG Ying, SONG Xinzhou, ZHANG Jiantao

Journal Article